reverse transcriptase m-mlv mutants superscript ii (Thermo Fisher)
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reverse transcriptase m-mlv mutants superscript ii
Reverse Transcriptase M Mlv Mutants Superscript Ii, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/m-mlv+superscript+ii+reverse+transcriptase/reverse+transcriptase+m+mlv+mutants+superscript+ii/us12258631-1083-17-28
Average 90 stars, based on 1 article reviews
Reverse Transcriptase M Mlv Mutants Superscript Ii, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/m-mlv+superscript+ii+reverse+transcriptase/reverse+transcriptase+m+mlv+mutants+superscript+ii/us12258631-1083-17-28
Average 90 stars, based on 1 article reviews
reverse transcriptase m-mlv mutants superscript ii - by Bioz Stars,
2026-09
90/100 stars
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Incubation:Article Title: CRISPR/Cas9-mediated activation of CDH1 suppresses metastasis of breast cancer in rats Article Snippet: 2.6.Gene expression analysis Total RNA was isolated from untreated and CRISPR/Cas9-treated MCF-7 cells using RNA Isolation System (Qiagen, GmbH, Germany). cDNA was synthesized by mixing 5 μg of the extracted RNA with 1 μg random 6-mer primers, 1 μM deoxyribonucleotides (dNTP), and 10 units of Article Title: Dominant-Negative Fas Mutation Is Reversed by Down-expression of c-FLIP Article Snippet: Cellular total RNA was extracted using the Article Title: Trastuzumab Induces Apoptosis and Cell Cycle Arrest in Triple-Negative Breast Cancer, Suggesting Repurposing Potential Article Snippet: The extracted RNA (4 μg) was incubated with 1 μM deoxyribonucleotides (dNTP), 10 U of Article Title: Activation of preexisting T cell clones by targeted interleukin 2 therapy Article Snippet: RNA was extracted using the method of Chomczynski and Sacchi as described ( 11 , 12 ). cDNA synthesis was carried out using 1–3 μg of total RNA with oligo-dT and M-MLV SuperScript II reverse transcriptase (GIBCO/BRL) in a total volume of 50 μl of 1× Article Title: Identification of the novel membrane-associated protein AgK114 on hamster keratinocytes recognized by a monoclonal antibody K114. Article Snippet: The cDNA was synthesized by using random primer and Article Title: ATM mutations are associated with inactivation of the ARF-TP53 tumor suppressor pathway in diffuse large B-cell lymphoma. Article Snippet: The ATM serine-threonine kinase plays a central role in the cellular response to DNA damage.. Germ-line mutations in the ATM gene cause ataxia-telangiectasia (A-T), a multisystem disorder associated with predisposition to lymphoma and acute leukemia.. Moreover, somatic ATM mutations have been identified in T-cell prolymphocytic leukemia, mantle cell lymphoma, and B-cell chronic lymphocytic leukemia. Article Title: Vascular endothelial growth factor (VEGF) is expressed by neoplastic Hodgkin-Reed-Sternberg cells in Hodgkin's disease. Article Snippet: Vascular endothelial growth factor (VEGF) is involved in tumour angiogenesis, an important process for the growth and metastatic potential of solid tumours.. Numerous studies have demonstrated up-regulation of VEGF at both mRNA and protein level in various tumours and a correlation with advanced stage and prognosis has been demonstrated in some cases.. Limited information exists about its role in lymphoid malignancies and in particular, Hodgkin’s disease. Article Title: Nestin-positive mesenchymal stem cells favour the astroglial lineage in neural progenitors and stem cells by releasing active BMP4 Article Snippet: The cDNA synthesis was carried out using Reverse Transcription:Article Title: CRISPR/Cas9-mediated activation of CDH1 suppresses metastasis of breast cancer in rats Article Snippet: 2.6.Gene expression analysis Total RNA was isolated from untreated and CRISPR/Cas9-treated MCF-7 cells using RNA Isolation System (Qiagen, GmbH, Germany). cDNA was synthesized by mixing 5 μg of the extracted RNA with 1 μg random 6-mer primers, 1 μM deoxyribonucleotides (dNTP), and 10 units of Article Title: Dominant-Negative Fas Mutation Is Reversed by Down-expression of c-FLIP Article Snippet: Cellular total RNA was extracted using the Article Title: Trastuzumab Induces Apoptosis and Cell Cycle Arrest in Triple-Negative Breast Cancer, Suggesting Repurposing Potential Article Snippet: The extracted RNA (4 μg) was incubated with 1 μM deoxyribonucleotides (dNTP), 10 U of Article Title: Activation of preexisting T cell clones by targeted interleukin 2 therapy Article Snippet: RNA was extracted using the method of Chomczynski and Sacchi as described ( 11 , 12 ). cDNA synthesis was carried out using 1–3 μg of total RNA with oligo-dT and M-MLV SuperScript II reverse transcriptase (GIBCO/BRL) in a total volume of 50 μl of 1× Article Title: Identification of the novel membrane-associated protein AgK114 on hamster keratinocytes recognized by a monoclonal antibody K114. Article Snippet: The cDNA was synthesized by using random primer and Article Title: ATM mutations are associated with inactivation of the ARF-TP53 tumor suppressor pathway in diffuse large B-cell lymphoma. Article Snippet: The ATM serine-threonine kinase plays a central role in the cellular response to DNA damage.. Germ-line mutations in the ATM gene cause ataxia-telangiectasia (A-T), a multisystem disorder associated with predisposition to lymphoma and acute leukemia.. Moreover, somatic ATM mutations have been identified in T-cell prolymphocytic leukemia, mantle cell lymphoma, and B-cell chronic lymphocytic leukemia. Article Title: Vascular endothelial growth factor (VEGF) is expressed by neoplastic Hodgkin-Reed-Sternberg cells in Hodgkin's disease. Article Snippet: Vascular endothelial growth factor (VEGF) is involved in tumour angiogenesis, an important process for the growth and metastatic potential of solid tumours.. Numerous studies have demonstrated up-regulation of VEGF at both mRNA and protein level in various tumours and a correlation with advanced stage and prognosis has been demonstrated in some cases.. Limited information exists about its role in lymphoid malignancies and in particular, Hodgkin’s disease. Article Title: Nestin-positive mesenchymal stem cells favour the astroglial lineage in neural progenitors and stem cells by releasing active BMP4 Article Snippet: The cDNA synthesis was carried out using Expressing:Article Title: CRISPR/Cas9-mediated activation of CDH1 suppresses metastasis of breast cancer in rats Article Snippet: 2.6.Gene expression analysis Total RNA was isolated from untreated and CRISPR/Cas9-treated MCF-7 cells using RNA Isolation System (Qiagen, GmbH, Germany). cDNA was synthesized by mixing 5 μg of the extracted RNA with 1 μg random 6-mer primers, 1 μM deoxyribonucleotides (dNTP), and 10 units of Article Title: Dominant-Negative Fas Mutation Is Reversed by Down-expression of c-FLIP Article Snippet: Cellular total RNA was extracted using the Article Title: Trastuzumab Induces Apoptosis and Cell Cycle Arrest in Triple-Negative Breast Cancer, Suggesting Repurposing Potential Article Snippet: The extracted RNA (4 μg) was incubated with 1 μM deoxyribonucleotides (dNTP), 10 U of Article Title: Activation of preexisting T cell clones by targeted interleukin 2 therapy Article Snippet: RNA was extracted using the method of Chomczynski and Sacchi as described ( 11 , 12 ). cDNA synthesis was carried out using 1–3 μg of total RNA with oligo-dT and M-MLV SuperScript II reverse transcriptase (GIBCO/BRL) in a total volume of 50 μl of 1× Article Title: Identification of the novel membrane-associated protein AgK114 on hamster keratinocytes recognized by a monoclonal antibody K114. Article Snippet: The cDNA was synthesized by using random primer and Article Title: ATM mutations are associated with inactivation of the ARF-TP53 tumor suppressor pathway in diffuse large B-cell lymphoma. Article Snippet: The ATM serine-threonine kinase plays a central role in the cellular response to DNA damage.. Germ-line mutations in the ATM gene cause ataxia-telangiectasia (A-T), a multisystem disorder associated with predisposition to lymphoma and acute leukemia.. Moreover, somatic ATM mutations have been identified in T-cell prolymphocytic leukemia, mantle cell lymphoma, and B-cell chronic lymphocytic leukemia. Article Title: Vascular endothelial growth factor (VEGF) is expressed by neoplastic Hodgkin-Reed-Sternberg cells in Hodgkin's disease. Article Snippet: Vascular endothelial growth factor (VEGF) is involved in tumour angiogenesis, an important process for the growth and metastatic potential of solid tumours.. Numerous studies have demonstrated up-regulation of VEGF at both mRNA and protein level in various tumours and a correlation with advanced stage and prognosis has been demonstrated in some cases.. Limited information exists about its role in lymphoid malignancies and in particular, Hodgkin’s disease. Article Title: Nestin-positive mesenchymal stem cells favour the astroglial lineage in neural progenitors and stem cells by releasing active BMP4 Article Snippet: The cDNA synthesis was carried out using Isolation:Article Title: CRISPR/Cas9-mediated activation of CDH1 suppresses metastasis of breast cancer in rats Article Snippet: 2.6.Gene expression analysis Total RNA was isolated from untreated and CRISPR/Cas9-treated MCF-7 cells using RNA Isolation System (Qiagen, GmbH, Germany). cDNA was synthesized by mixing 5 μg of the extracted RNA with 1 μg random 6-mer primers, 1 μM deoxyribonucleotides (dNTP), and 10 units of Article Title: Dominant-Negative Fas Mutation Is Reversed by Down-expression of c-FLIP Article Snippet: Cellular total RNA was extracted using the Article Title: Trastuzumab Induces Apoptosis and Cell Cycle Arrest in Triple-Negative Breast Cancer, Suggesting Repurposing Potential Article Snippet: The extracted RNA (4 μg) was incubated with 1 μM deoxyribonucleotides (dNTP), 10 U of Article Title: Activation of preexisting T cell clones by targeted interleukin 2 therapy Article Snippet: RNA was extracted using the method of Chomczynski and Sacchi as described ( 11 , 12 ). cDNA synthesis was carried out using 1–3 μg of total RNA with oligo-dT and M-MLV SuperScript II reverse transcriptase (GIBCO/BRL) in a total volume of 50 μl of 1× Article Title: Identification of the novel membrane-associated protein AgK114 on hamster keratinocytes recognized by a monoclonal antibody K114. Article Snippet: The cDNA was synthesized by using random primer and Article Title: ATM mutations are associated with inactivation of the ARF-TP53 tumor suppressor pathway in diffuse large B-cell lymphoma. Article Snippet: The ATM serine-threonine kinase plays a central role in the cellular response to DNA damage.. Germ-line mutations in the ATM gene cause ataxia-telangiectasia (A-T), a multisystem disorder associated with predisposition to lymphoma and acute leukemia.. Moreover, somatic ATM mutations have been identified in T-cell prolymphocytic leukemia, mantle cell lymphoma, and B-cell chronic lymphocytic leukemia. Article Title: Vascular endothelial growth factor (VEGF) is expressed by neoplastic Hodgkin-Reed-Sternberg cells in Hodgkin's disease. Article Snippet: Vascular endothelial growth factor (VEGF) is involved in tumour angiogenesis, an important process for the growth and metastatic potential of solid tumours.. Numerous studies have demonstrated up-regulation of VEGF at both mRNA and protein level in various tumours and a correlation with advanced stage and prognosis has been demonstrated in some cases.. Limited information exists about its role in lymphoid malignancies and in particular, Hodgkin’s disease. Article Title: Nestin-positive mesenchymal stem cells favour the astroglial lineage in neural progenitors and stem cells by releasing active BMP4 Article Snippet: The cDNA synthesis was carried out using CRISPR:Article Title: CRISPR/Cas9-mediated activation of CDH1 suppresses metastasis of breast cancer in rats Article Snippet: 2.6.Gene expression analysis Total RNA was isolated from untreated and CRISPR/Cas9-treated MCF-7 cells using RNA Isolation System (Qiagen, GmbH, Germany). cDNA was synthesized by mixing 5 μg of the extracted RNA with 1 μg random 6-mer primers, 1 μM deoxyribonucleotides (dNTP), and 10 units of Article Title: Dominant-Negative Fas Mutation Is Reversed by Down-expression of c-FLIP Article Snippet: Cellular total RNA was extracted using the Article Title: Trastuzumab Induces Apoptosis and Cell Cycle Arrest in Triple-Negative Breast Cancer, Suggesting Repurposing Potential Article Snippet: The extracted RNA (4 μg) was incubated with 1 μM deoxyribonucleotides (dNTP), 10 U of Article Title: Activation of preexisting T cell clones by targeted interleukin 2 therapy Article Snippet: RNA was extracted using the method of Chomczynski and Sacchi as described ( 11 , 12 ). cDNA synthesis was carried out using 1–3 μg of total RNA with oligo-dT and M-MLV SuperScript II reverse transcriptase (GIBCO/BRL) in a total volume of 50 μl of 1× Article Title: Identification of the novel membrane-associated protein AgK114 on hamster keratinocytes recognized by a monoclonal antibody K114. Article Snippet: The cDNA was synthesized by using random primer and Article Title: ATM mutations are associated with inactivation of the ARF-TP53 tumor suppressor pathway in diffuse large B-cell lymphoma. Article Snippet: The ATM serine-threonine kinase plays a central role in the cellular response to DNA damage.. Germ-line mutations in the ATM gene cause ataxia-telangiectasia (A-T), a multisystem disorder associated with predisposition to lymphoma and acute leukemia.. Moreover, somatic ATM mutations have been identified in T-cell prolymphocytic leukemia, mantle cell lymphoma, and B-cell chronic lymphocytic leukemia. Article Title: Vascular endothelial growth factor (VEGF) is expressed by neoplastic Hodgkin-Reed-Sternberg cells in Hodgkin's disease. Article Snippet: Vascular endothelial growth factor (VEGF) is involved in tumour angiogenesis, an important process for the growth and metastatic potential of solid tumours.. Numerous studies have demonstrated up-regulation of VEGF at both mRNA and protein level in various tumours and a correlation with advanced stage and prognosis has been demonstrated in some cases.. Limited information exists about its role in lymphoid malignancies and in particular, Hodgkin’s disease. Article Title: Nestin-positive mesenchymal stem cells favour the astroglial lineage in neural progenitors and stem cells by releasing active BMP4 Article Snippet: The cDNA synthesis was carried out using Synthesized:Article Title: CRISPR/Cas9-mediated activation of CDH1 suppresses metastasis of breast cancer in rats Article Snippet: 2.6.Gene expression analysis Total RNA was isolated from untreated and CRISPR/Cas9-treated MCF-7 cells using RNA Isolation System (Qiagen, GmbH, Germany). cDNA was synthesized by mixing 5 μg of the extracted RNA with 1 μg random 6-mer primers, 1 μM deoxyribonucleotides (dNTP), and 10 units of Article Title: Dominant-Negative Fas Mutation Is Reversed by Down-expression of c-FLIP Article Snippet: Cellular total RNA was extracted using the Article Title: Trastuzumab Induces Apoptosis and Cell Cycle Arrest in Triple-Negative Breast Cancer, Suggesting Repurposing Potential Article Snippet: The extracted RNA (4 μg) was incubated with 1 μM deoxyribonucleotides (dNTP), 10 U of Article Title: Activation of preexisting T cell clones by targeted interleukin 2 therapy Article Snippet: RNA was extracted using the method of Chomczynski and Sacchi as described ( 11 , 12 ). cDNA synthesis was carried out using 1–3 μg of total RNA with oligo-dT and M-MLV SuperScript II reverse transcriptase (GIBCO/BRL) in a total volume of 50 μl of 1× Article Title: Identification of the novel membrane-associated protein AgK114 on hamster keratinocytes recognized by a monoclonal antibody K114. Article Snippet: The cDNA was synthesized by using random primer and Article Title: ATM mutations are associated with inactivation of the ARF-TP53 tumor suppressor pathway in diffuse large B-cell lymphoma. Article Snippet: The ATM serine-threonine kinase plays a central role in the cellular response to DNA damage.. Germ-line mutations in the ATM gene cause ataxia-telangiectasia (A-T), a multisystem disorder associated with predisposition to lymphoma and acute leukemia.. Moreover, somatic ATM mutations have been identified in T-cell prolymphocytic leukemia, mantle cell lymphoma, and B-cell chronic lymphocytic leukemia. Article Title: Vascular endothelial growth factor (VEGF) is expressed by neoplastic Hodgkin-Reed-Sternberg cells in Hodgkin's disease. Article Snippet: Vascular endothelial growth factor (VEGF) is involved in tumour angiogenesis, an important process for the growth and metastatic potential of solid tumours.. Numerous studies have demonstrated up-regulation of VEGF at both mRNA and protein level in various tumours and a correlation with advanced stage and prognosis has been demonstrated in some cases.. Limited information exists about its role in lymphoid malignancies and in particular, Hodgkin’s disease. Article Title: Nestin-positive mesenchymal stem cells favour the astroglial lineage in neural progenitors and stem cells by releasing active BMP4 Article Snippet: The cDNA synthesis was carried out using Plasmid Preparation:Article Title: CRISPR/Cas9-mediated activation of CDH1 suppresses metastasis of breast cancer in rats Article Snippet: 2.6.Gene expression analysis Total RNA was isolated from untreated and CRISPR/Cas9-treated MCF-7 cells using RNA Isolation System (Qiagen, GmbH, Germany). cDNA was synthesized by mixing 5 μg of the extracted RNA with 1 μg random 6-mer primers, 1 μM deoxyribonucleotides (dNTP), and 10 units of Article Title: Dominant-Negative Fas Mutation Is Reversed by Down-expression of c-FLIP Article Snippet: Cellular total RNA was extracted using the Article Title: Trastuzumab Induces Apoptosis and Cell Cycle Arrest in Triple-Negative Breast Cancer, Suggesting Repurposing Potential Article Snippet: The extracted RNA (4 μg) was incubated with 1 μM deoxyribonucleotides (dNTP), 10 U of Article Title: Activation of preexisting T cell clones by targeted interleukin 2 therapy Article Snippet: RNA was extracted using the method of Chomczynski and Sacchi as described ( 11 , 12 ). cDNA synthesis was carried out using 1–3 μg of total RNA with oligo-dT and M-MLV SuperScript II reverse transcriptase (GIBCO/BRL) in a total volume of 50 μl of 1× Article Title: Identification of the novel membrane-associated protein AgK114 on hamster keratinocytes recognized by a monoclonal antibody K114. Article Snippet: The cDNA was synthesized by using random primer and Article Title: ATM mutations are associated with inactivation of the ARF-TP53 tumor suppressor pathway in diffuse large B-cell lymphoma. Article Snippet: The ATM serine-threonine kinase plays a central role in the cellular response to DNA damage.. Germ-line mutations in the ATM gene cause ataxia-telangiectasia (A-T), a multisystem disorder associated with predisposition to lymphoma and acute leukemia.. Moreover, somatic ATM mutations have been identified in T-cell prolymphocytic leukemia, mantle cell lymphoma, and B-cell chronic lymphocytic leukemia. Article Title: Vascular endothelial growth factor (VEGF) is expressed by neoplastic Hodgkin-Reed-Sternberg cells in Hodgkin's disease. Article Snippet: Vascular endothelial growth factor (VEGF) is involved in tumour angiogenesis, an important process for the growth and metastatic potential of solid tumours.. Numerous studies have demonstrated up-regulation of VEGF at both mRNA and protein level in various tumours and a correlation with advanced stage and prognosis has been demonstrated in some cases.. Limited information exists about its role in lymphoid malignancies and in particular, Hodgkin’s disease. Article Title: Nestin-positive mesenchymal stem cells favour the astroglial lineage in neural progenitors and stem cells by releasing active BMP4 Article Snippet: The cDNA synthesis was carried out using RNA Extraction:Article Title: CRISPR/Cas9-mediated activation of CDH1 suppresses metastasis of breast cancer in rats Article Snippet: 2.6.Gene expression analysis Total RNA was isolated from untreated and CRISPR/Cas9-treated MCF-7 cells using RNA Isolation System (Qiagen, GmbH, Germany). cDNA was synthesized by mixing 5 μg of the extracted RNA with 1 μg random 6-mer primers, 1 μM deoxyribonucleotides (dNTP), and 10 units of Article Title: Dominant-Negative Fas Mutation Is Reversed by Down-expression of c-FLIP Article Snippet: Cellular total RNA was extracted using the Article Title: Trastuzumab Induces Apoptosis and Cell Cycle Arrest in Triple-Negative Breast Cancer, Suggesting Repurposing Potential Article Snippet: The extracted RNA (4 μg) was incubated with 1 μM deoxyribonucleotides (dNTP), 10 U of Article Title: Activation of preexisting T cell clones by targeted interleukin 2 therapy Article Snippet: RNA was extracted using the method of Chomczynski and Sacchi as described ( 11 , 12 ). cDNA synthesis was carried out using 1–3 μg of total RNA with oligo-dT and M-MLV SuperScript II reverse transcriptase (GIBCO/BRL) in a total volume of 50 μl of 1× Article Title: Identification of the novel membrane-associated protein AgK114 on hamster keratinocytes recognized by a monoclonal antibody K114. Article Snippet: The cDNA was synthesized by using random primer and Article Title: ATM mutations are associated with inactivation of the ARF-TP53 tumor suppressor pathway in diffuse large B-cell lymphoma. Article Snippet: The ATM serine-threonine kinase plays a central role in the cellular response to DNA damage.. Germ-line mutations in the ATM gene cause ataxia-telangiectasia (A-T), a multisystem disorder associated with predisposition to lymphoma and acute leukemia.. Moreover, somatic ATM mutations have been identified in T-cell prolymphocytic leukemia, mantle cell lymphoma, and B-cell chronic lymphocytic leukemia. Article Title: Vascular endothelial growth factor (VEGF) is expressed by neoplastic Hodgkin-Reed-Sternberg cells in Hodgkin's disease. Article Snippet: Vascular endothelial growth factor (VEGF) is involved in tumour angiogenesis, an important process for the growth and metastatic potential of solid tumours.. Numerous studies have demonstrated up-regulation of VEGF at both mRNA and protein level in various tumours and a correlation with advanced stage and prognosis has been demonstrated in some cases.. Limited information exists about its role in lymphoid malignancies and in particular, Hodgkin’s disease. Article Title: Nestin-positive mesenchymal stem cells favour the astroglial lineage in neural progenitors and stem cells by releasing active BMP4 Article Snippet: The cDNA synthesis was carried out using Generated:Article Title: CRISPR/Cas9-mediated activation of CDH1 suppresses metastasis of breast cancer in rats Article Snippet: 2.6.Gene expression analysis Total RNA was isolated from untreated and CRISPR/Cas9-treated MCF-7 cells using RNA Isolation System (Qiagen, GmbH, Germany). cDNA was synthesized by mixing 5 μg of the extracted RNA with 1 μg random 6-mer primers, 1 μM deoxyribonucleotides (dNTP), and 10 units of Article Title: Dominant-Negative Fas Mutation Is Reversed by Down-expression of c-FLIP Article Snippet: Cellular total RNA was extracted using the Article Title: Trastuzumab Induces Apoptosis and Cell Cycle Arrest in Triple-Negative Breast Cancer, Suggesting Repurposing Potential Article Snippet: The extracted RNA (4 μg) was incubated with 1 μM deoxyribonucleotides (dNTP), 10 U of Article Title: Activation of preexisting T cell clones by targeted interleukin 2 therapy Article Snippet: RNA was extracted using the method of Chomczynski and Sacchi as described ( 11 , 12 ). cDNA synthesis was carried out using 1–3 μg of total RNA with oligo-dT and M-MLV SuperScript II reverse transcriptase (GIBCO/BRL) in a total volume of 50 μl of 1× Article Title: Identification of the novel membrane-associated protein AgK114 on hamster keratinocytes recognized by a monoclonal antibody K114. Article Snippet: The cDNA was synthesized by using random primer and Article Title: ATM mutations are associated with inactivation of the ARF-TP53 tumor suppressor pathway in diffuse large B-cell lymphoma. Article Snippet: The ATM serine-threonine kinase plays a central role in the cellular response to DNA damage.. Germ-line mutations in the ATM gene cause ataxia-telangiectasia (A-T), a multisystem disorder associated with predisposition to lymphoma and acute leukemia.. Moreover, somatic ATM mutations have been identified in T-cell prolymphocytic leukemia, mantle cell lymphoma, and B-cell chronic lymphocytic leukemia. Article Title: Vascular endothelial growth factor (VEGF) is expressed by neoplastic Hodgkin-Reed-Sternberg cells in Hodgkin's disease. Article Snippet: Vascular endothelial growth factor (VEGF) is involved in tumour angiogenesis, an important process for the growth and metastatic potential of solid tumours.. Numerous studies have demonstrated up-regulation of VEGF at both mRNA and protein level in various tumours and a correlation with advanced stage and prognosis has been demonstrated in some cases.. Limited information exists about its role in lymphoid malignancies and in particular, Hodgkin’s disease. Article Title: Nestin-positive mesenchymal stem cells favour the astroglial lineage in neural progenitors and stem cells by releasing active BMP4 Article Snippet: The cDNA synthesis was carried out using Polymerase Chain Reaction:Article Title: CRISPR/Cas9-mediated activation of CDH1 suppresses metastasis of breast cancer in rats Article Snippet: 2.6.Gene expression analysis Total RNA was isolated from untreated and CRISPR/Cas9-treated MCF-7 cells using RNA Isolation System (Qiagen, GmbH, Germany). cDNA was synthesized by mixing 5 μg of the extracted RNA with 1 μg random 6-mer primers, 1 μM deoxyribonucleotides (dNTP), and 10 units of Article Title: Dominant-Negative Fas Mutation Is Reversed by Down-expression of c-FLIP Article Snippet: Cellular total RNA was extracted using the Article Title: Trastuzumab Induces Apoptosis and Cell Cycle Arrest in Triple-Negative Breast Cancer, Suggesting Repurposing Potential Article Snippet: The extracted RNA (4 μg) was incubated with 1 μM deoxyribonucleotides (dNTP), 10 U of Article Title: Activation of preexisting T cell clones by targeted interleukin 2 therapy Article Snippet: RNA was extracted using the method of Chomczynski and Sacchi as described ( 11 , 12 ). cDNA synthesis was carried out using 1–3 μg of total RNA with oligo-dT and M-MLV SuperScript II reverse transcriptase (GIBCO/BRL) in a total volume of 50 μl of 1× Article Title: Identification of the novel membrane-associated protein AgK114 on hamster keratinocytes recognized by a monoclonal antibody K114. Article Snippet: The cDNA was synthesized by using random primer and Article Title: ATM mutations are associated with inactivation of the ARF-TP53 tumor suppressor pathway in diffuse large B-cell lymphoma. Article Snippet: The ATM serine-threonine kinase plays a central role in the cellular response to DNA damage.. Germ-line mutations in the ATM gene cause ataxia-telangiectasia (A-T), a multisystem disorder associated with predisposition to lymphoma and acute leukemia.. Moreover, somatic ATM mutations have been identified in T-cell prolymphocytic leukemia, mantle cell lymphoma, and B-cell chronic lymphocytic leukemia. Article Title: Vascular endothelial growth factor (VEGF) is expressed by neoplastic Hodgkin-Reed-Sternberg cells in Hodgkin's disease. Article Snippet: Vascular endothelial growth factor (VEGF) is involved in tumour angiogenesis, an important process for the growth and metastatic potential of solid tumours.. Numerous studies have demonstrated up-regulation of VEGF at both mRNA and protein level in various tumours and a correlation with advanced stage and prognosis has been demonstrated in some cases.. Limited information exists about its role in lymphoid malignancies and in particular, Hodgkin’s disease. Article Title: Nestin-positive mesenchymal stem cells favour the astroglial lineage in neural progenitors and stem cells by releasing active BMP4 Article Snippet: The cDNA synthesis was carried out using |